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Mouse IL-6 ELISA Kit

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Cat# ELM-IL6-1

Size : 1PlateKit

Brand : RayBiotech


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Mouse IL-6 ELISA Kit Standard CurveMouse IL-6 ELISA KitMouse IL-6 ELISA KitMouse IL-6 ELISA KitMouse IL-6 ELISA KitELM-IL6 was used to measure plasma IL-6 in diet-induced obese mice following hepatocyte-specific DPP4 silencing. Figure adapted from Ghorpade et al., Nature, 2018.ELM-IL6 was used to measure serum IL-6 in high-fat diet–fed mice with adipocyte-specific TRPM7 deletion. Figure adapted from Zhong et al., Nature Communications, 2023.ELM-IL6 was used to measure plasma IL-6 in HT1080 tumor-bearing mice treated with selpercatinib. Figure adapted from Khatri et al., Cancer Letters, 2025.ELM-IL6 was used to measure IL-6 in colon tissue homogenates in a DSS-induced colitis mouse model with dietary WKB intervention. Figure adapted from Sun et al., Journal of Translational Medicine, 2025.RayBiotech’s ELM-TNFα, ELM-IL1β, ELM-IL6, and ELM-IL10 ELISA kits were used to measure serum cytokine levels in high-fat diet–induced pancreatic dysfunction in mice. Figure adapted from Mitra et al., BBA – Molecular Basis of Disease, 2024.ELM-IL6 was used to measure liver and plasma IL-6 in high-fat diet–fed liver-specific matrin-3 knockout mice. Figure adapted from Cheng et al., Molecular Metabolism, 2024.Mouse IL-6 ELISA KitMouse IL-6 ELISA KitMouse IL-6 ELISA Kit5 Plate Kit Packaging (one box)

Mouse IL-6 ELISA Kit Standard Curve

Mouse IL-6 ELISA Kit

The RayBiotech Mouse IL-6 ELISA Kit is a pre-coated immunoassay for the measurement of Interleukin 6 in mouse cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesMouse
Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 600 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P08505
Gene Id
16193
Gene Symbols
IL6, IL-6
Protein Name / Synonyms
Interleukin-6 (IL-6 / IL6) (B-cell hybridoma growth factor) (Interleukin HP-1)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Cancer, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Introduction

The RayBio® Mouse IL-6 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Interleukin 6 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

Interleukin 6 is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. It acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-α and IL-1, and activation of IL-1Rα and IL-10.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Pre-coated microplates for better reproducibility and efficiency
  • Fully optimized for serum, plasma, cell culture medium, and lysates
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of multiplex array data

Standard Curves

Mouse IL-6 ELISA Standard Curve

Mouse IL-6 ELISA Standard Dilution Series

Spiking & Recovery Results

Mouse IL-6 ELISA spiking and recovery data results

Linearity Results

Mouse IL-6 ELISA linearity data results

Typical Data

ELM-IL6-1 was used to measure plasma IL-6 in diet-induced obese mice following hepatocyte-specific DPP4 silencing. Figure adapted from Ghorpade et al., Nature, 2018.

ELM-IL6 was used to measure serum IL-6 in high-fat diet–fed mice with adipocyte-specific TRPM7 deletion. Figure adapted from Zhong et al., Nature Communications, 2023.

ELM-IL6 was used to measure plasma IL-6 in HT1080 tumor-bearing mice treated with selpercatinib. Figure adapted from Khatri et al., Cancer Letters, 2025.

ELM-IL6 was used to measure IL-6 in colon tissue homogenates in a DSS-induced colitis mouse model with dietary WKB intervention. Figure adapted from Sun et al., Journal of Translational Medicine, 2025.

RayBiotech’s ELM-TNFα, ELM-IL1β, ELM-IL6, and ELM-IL10 ELISA kits were used to measure serum cytokine levels in high-fat diet–induced pancreatic dysfunction in mice. Figure adapted from Mitra et al., BBA – Molecular Basis of Disease, 2024.

ELM-IL6 was used to measure liver and plasma IL-6 in high-fat diet–fed liver-specific matrin-3 knockout mice. Figure adapted from Cheng et al., Molecular Metabolism, 2024.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

citations

  • Hepatocyte-secreted DPP4 in obesity promotes adipose inflammation and insulin resistance

    Year:
    2018
    Journal Name:
    Nature
    PubMed ID:
    29562231

  • Rescue of maternal immune activation-induced behavioral abnormalities in adult mouse offspring by pathogen-activated maternal Treg cells

    Year:
    2021
    Journal Name:
    Nature Neuroscience
    PubMed ID:
    33859437

  • Adipose-specific deletion of the cation channel TRPM7 inhibits TAK1 kinase-dependent inflammation and obesity in male mice

    Species:
    Mouse
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Nature Communications

  • Oral Microalgae-Nano Integrated System against Radiation-Induced Injury

    Species:
    Mouse
    Sample Type:
    small intestinal tissue
    Year:
    2023
    Journal Name:
    Acs Nano

  • Selpercatinib mitigates cancer cachexia independent of anti-tumor activity in the HT1080 tumor model

    Year:
    2025
    Journal Name:
    Cancer Letters
    PubMed ID:
    39778760

  • WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota

    Year:
    2025
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    39838431

  • Shaoyao decoction alleviates DSS-induced colitis by inhibiting IL-17a-mediated polarization of M1 macrophages

    Year:
    2025
    Journal Name:
    Journal of Ethnopharmacology
    PubMed ID:
    39427735

  • A potential therapeutic effect of sea cucumber Holothuria polii extract during the intestinal phase of experimental trichinellosis

    Year:
    2025
    Journal Name:
    J Parasit Dis
    PubMed ID:
    39975624

  • Metformin instigates cellular autophagy to ameliorate high-fat diet-induced pancreatic inflammation and fibrosis/EMT in mice

    Year:
    2024
    Journal Name:
    Biochim Biophys Acta Mol Basis Dis
    PubMed ID:
    38901652

  • Liver matrin-3 protects mice against hepatic steatosis and stress response via constitutive androstane receptor

    Year:
    2024
    Journal Name:
    Mol Metab
    PubMed ID:
    38936659

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