Rhodamine 123
Cat# 70010
Size : 50MG
Brand : Biotium
Rhodamine 123[62669-70-9]
Cellular Stains
Troubleshooting Tips for Fluorescence Staining
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Fluorescence Bleed-Through and Cross-Talk
Rhodamine 123
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.
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Product Description
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential. Please also see Dihydrorhodamine 123 and its HCl salt (10055 and 10056).
- λEx/λEm (MeOH) = 505/534 nm
- ε (505 nm, MeOH) = 97,000
- Orange red solid soluble in DMSO or DMF
- Store at 4°C and protect from light, especially in solution
- C21H17ClN2O3
- MW: 381
- [62669-70-9]
Technology
Tech Tips
Product Attributes
CAS number
62669-70-9
Size
50 mg
Probe cellular localization
Mitochondria
For live or fixed cells
For live/intact cells
Assay type/options
Real-time imaging
Cell permeability
Membrane permeant
Apoptosis/viability marker
Mitochondrial potential
Potential dependence
Mitochondrial potential-dependent
Colors
Red
Excitation/Emission
505/534 nm
Documents, Protocols, SDS and COA
Protocols
COA
Request the Certificate of AnalysisReferences
1. Cytometry 17, 50 (1994).
2. Science 218, 1117 (1982).
3. J Cell Biol 88, 526 (1981).
Citations
1. Cytometry 17, 50 (1994).
2. Science 218, 1117 (1982).
3. J Cell Biol 88, 526 (1981).
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