SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1, Histone H3-K9 Methyltransferase 1, H3-K9-HMTase 1, Su(var)3-9 Homolog 1, KMT1A, Histone-lysine N-methyltransferase SUV39H1, Position-effect Variegation 3-9 Homolog, Lysine N-methyltransferase 1A) (Biotin)

Cat# S8950-01A-Biotin-200ul

Size : 200ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Host
mouse

Conjugate
Biotin

Isotype
IgG1

Clone Number
10i75

Grade
Affinity Purified

Applications
E WB

Shipping Temp
Blue Ice

Storage Temp
-20°C

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Product Type
Mab

Isotype
IgG1

Clone No
10i75

Host
mouse

Source
human

Concentration
As reported

Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Purity
Purified by Protein G affinity chromatography.

Immunogen
Purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Specificity
Recognizes human SUV39H1. Species Crossreactivity


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.