CD45 / LCA Rat Monoclonal Antibody [Clone ID: I3/2.3]

Specifications

Product Data
Clone Name	I3/2.3
Applications	FC, IP
Recommended Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.4,5) Immunoprecipitation. (Ref.3,5)
Reactivities	Mouse
Host	Rat
Isotype	IgG2b
Clonality	Monoclonal
Specificity	This antibody recognizes a framework epitope present on all CD45 isoforms. (Ref.3-6)
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Biotin
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 while those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data