Glucose-6-phosphate is glucose phosphorylated at the sixth carbon, representing the first intracellular metabolite formed after glucose uptake. It serves as a substrate for multiple metabolic pathways including glycolysis, pentose phosphate pathway, and glycogen synthesis. Measuring G6P levels in serum, plasma, tissue extracts, or culture media is important for understanding metabolic fluxes and pathological conditions related to carbohydrate metabolism.

Applications

• Monitoring metabolic flux through glycolysis and pentose phosphate pathways
• Investigating oxidative stress and antioxidant capacity in clinical and research settings
• Studying metabolic alterations in diabetes, obesity, and related disorders
• Assessing plant and environmental stress responses via G6P levels
• High-throughput screening of biological samples in metabolic research

Advantages 

• Simple, rapid, and sensitive assay suitable for high-throughput analysis
• No separation or washing steps required, reducing assay time and complexity
• Compatible with multiple biological sample types including serum
• Linear detection range covers physiological and pathological concentrations

Glucose-6-phosphate assay kits provide a robust, sensitive, and efficient method for quantifying G6P in serum and other biological samples. By leveraging enzymatic oxidation coupled with colorimetric detection, these kits facilitate metabolic research and clinical investigations into carbohydrate metabolism and associated diseases. Their ease of use and compatibility with microplate readers make them well-suited for both routine and high-throughput applications.