HRP Chromogen for IHC - AEC
AEC (Aminoethyl carbazole) is one of the most used chromogens for immunohistochemistry (IHC) using horseradish peroxidase (HRP). HRP catalyzes the formation of a red precipitate from AEC.
The AEC is easy to use, simply incubate the slides for 5-10 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under microscope.
Since AEC is soluble in alcohol, samples should not be dehydrated after staining.
Slides can be mounted using an aqueous mounting medium (Simpo-Mount, NeoMount ...).
AEC can be used as a contrast stain with tissue pigments or for multiple labeling with other chromogens.
Name
AEC (Aminoethyl carbazole)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Red
Microscopy
Brightfield, Spectral Imaging
Mounting
Aqueous
Other properties
Contrast with Tissue Pigments, Multiple Labeling
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