HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 1054
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- Primary antibody 1200
- Protein/peptide 989
- Biochemicals 828
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- 48
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- WB 711
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- IP 102
- ICC 87
- FC 86
- IF 75
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- Chemiluminescence 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- ISH 1
- PCR 1
- Polyclonal 612
- 2G6 32
- 4G5 32
- 1F8 31
- 1G1 31
- 1G5 31
- 10i46 26
- 13H261 22
- 3H1166 22
- Monoclonal 18
- M-A261 12
- HLy9.1.25 8
- 1626CT702.263.21 6
- 1A10A10 5
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- 10.1F8 4
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- 2E4 4
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- 6.1H3.D9.C6 4
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- 6.8E7.A7.A1 4
- 8C7 4
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- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- Not Applicable 4
- 6D23 3
- 12K274 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- RB21227 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
- Recombinant 1
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