PCR Mix

PCR Mix



PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR

PCR master mix contains allcomponents for PCR, except DNA template and primers :

Taq DNA polymerase
A DNA polymerase that is heat resistant, so that it can remain intact during the DNA denaturation process and makes the apmlification of the target DNA sequence

Deoxynucleoside triphosphates (dNTPs)
dNTPs consist of four basic nucleotides : dATP, dCTP, dGTP, and dTTP. dNTPs are building blocks of new DNA strands. These four nucleotides are typically added to the PCR reaction in equimolar amounts for optimal base incorporation. In common PCR applications, the recommended final concentration of each dNTP is generally 0.2 mM.

Magnesium ion (Mg2+)
Magnesium ion (Mg2+) functions as a cofactor for activity of DNA polymerases by enabling incorporation of dNTPs during polymerization. The magnesium ions at the enzyme's active site catalyze phosphodiester bond formation between the 3′-OH of a primer and the phosphate group of a dNTP. ). In addition, Mg2+ facilitates formation of the complex between the primers and DNA templates by stabilizing negative charges on their phosphate backbones. Magnesium ion (Mg2+) are present in master mix as MgCl2 solution.

Buffer
PCR is carried out in a buffer that provides a suitable chemical environment for activity of DNA polymerase. Generally a 10-50 mM Tris/HCl buffer with a pH above 8.0 (typically 8.3-8.8). KCl can be added to facilitate primer annealing, but shouldn't be higher than 50 mM as this may inhibit polymerase.


We offer a wide range of PCR master mix. Please find below a selection with main features :

Name Taq Mix AceTaq Master Mix EsayTaq PCR SuperMix
Product code NB-03-0088 and NB-03-0089 P412 AS111
Type Taq polymerase Taq polymerase Taq polymerase
Speed 0.9-1.2 kb/min 1 kb/min 1-2 kb/min
Amplicon size < 5kb < 5kb < 4kb
5'-3' exonuclease activity Yes Yes Yes
3'-5' exonuclease activity No Yes No
HotStart No Yes No


Search result : 520 product found

Refine your search :

RUOCE / IVD
  • Unconjugated 89
  • polymerase 11
  • human 2
  • mouse 2
  • rat 2
  • pcr products 338
  • Buffers and reagents 80
  • Biochemicals 42
  • kit 28
  • Polymerase 11
  • Protein/peptide 9
  • Genomic DNA 3
  • Inhibitor/Antagonist/Agonist 3
  • Other products 2
  • Restriction enzyme 2
  • Service 1
  • cDNA 1
  • horse 5
  • bacteria 3
  • rabbit 2
  • PCR 172
  • 5G4 2
APPLY FILTERS
REINITIALIZE


Cat#
Description
Cond.
Price Bef. VAT
P112-02
 15×1ml 
P111-01
 5×1ml 
P112-01
 5×1ml 
P112-03
 50×1ml 
P515-02
 5×1ml 
NB-54-0022-03
 15×1ml 
NB-54-0022-02
 5×1ml 
NB-54-0022-01
 1ml 
NB-54-0239-01
 5ml 
NB-54-0239-02
 10×5ml 
NB-54-0023-03
 15×1ml 
AS111-03
 15×1ml 
P111-02
 15×1ml 
P515-01
 1ml 
P222-01
 5ml 
P510-02
 5×1ml