Weigert-Van Gieson stain

Weigert-Van Gieson stain


Weigert-Van Gieson stain is a method based on the affinity towards elastic fibers displayed by resorcin fuchsin, a precipitate resulting from a reaction between resorcin and basic fuchsin and ferric chloride. Since this is not an absolutely specific method, other structures such as collagen and basal membranes might be stained. Therefore it is essential to differentiate carefully in order to obtain a selective marked staining of elastic fibers. Weigert's “long method” differs from “quick method” in both staining mixture and procedure; it is more selective because staining mixture is less concentrated and incubation times are very long. Counterstaining with Van Gieson trichromic stain makes it possible to differentiate collagen from connectivum showing nuclei at the same time.

Here are examples of stains obtained with the Weigert-Van Gieson stain:
  • Nuclei: Black
  • Collagen: Different levels of red
  • Connectivum, erythrocytes: yellow

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