Extraction and purification of plasmidic DNA
A plasmid is a circular double-stranded DNA molecule. The plasmid is independent of chromosomal DNA and is capable of autonomous replication that is not essential for the survival of the cell. This replication takes place from the origin of replication of the plasmid. Plasmids occur naturally in prokaryotes (bacteria) and eukaryotes (yeast) but are also artificially modified for use as a tool in molecular biology. Different plasmids can co-exist in a cell (provided they are mutually compatible).
In nature, plasmids are pieces of DNA that can be transmitted by horizontal transfer from cell to cell. If it carries a gene of interest (e.g. resistance to an antibiotic), this is a definite advantage for the cells that possess it.
Researchers use plasmids to insert a DNA fragment of interest into the cell, thanks to cloning cassettes. This allows them to clone genes to express proteins or RNA and analyse them.
Frequently Asked Questions (FAQ)
Where can I find plasmidic dna products on the CliniSciences website?
plasmidic dna products are available in this section and can be sorted by product families or sub-categories, such as Miniprep, Miniprep - 96-well plate, Maxiprep. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the plasmidic dna category?
The plasmidic dna category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Maxiprep, Miniprep - 96-well plate, Midiprep sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a plasmidic dna product?
To find a plasmidic dna product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the plasmidic dna category?
Yes, CliniSciences distributes several product references in the plasmidic dna category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for plasmidic dna products?
Yes, you can request a quote for plasmidic dna products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a plasmidic dna product is available to order?
The availability of a plasmidic dna product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the plasmidic dna product I am looking for?
If the plasmidic dna product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all plasmidic dna products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.