MHC Class I H-2 Kk Mouse Monoclonal Antibody [Clone ID: 16-3-22S]

Specifications

Product Data
Clone Name	16-3-22S
Applications	FC
Recommended Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivities	Mouse
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	C3H.SW mouse splenocytes.
Specificity	This antibody reacts with the H-2Kk MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. (Ref.2) It does not react with other (e.g., b, d, q) haplotypes.
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P04223
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
Synonyms	H2-K1, H2-K, H-2K(K)
Reference Data