Siglec 1 (Sialic Acid Binding Ig-like Lectin 1, CD169, CD169 Antigen, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Sialic Acid-binding Ig-like Lectin 1, Sialic Acid-binding Ig-like Lectin-1, Sialoadhesin, Sialoadhesin Precursor, Siglec 1, SN) (HRP)

Siglec-1 cell surface antigen, also known as CD169, is expressed by subsets of cells of the macrophage/ monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes.

Suitable for use in Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested.

Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Porcine spleen

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Mab

IgG1

10B853 (3B11/11)

mouse

porcine

As Reported

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purified by Protein G affinity chromatography from tissue culture supernatant.

Porcine alveolar macrophages

Recognizes porcine Siglec-1 at ~190kD in alveolar macrophage extracts under non-reducing conditions.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.