LYZ High Sensitivity ELISA Kit (Human)

Cat# OKCD01349

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsPrintable datasheet for LZM ELISA kit (Human) (OKCD01349)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)79-97%90-98%80-101%85-103%
EDTA Plasma (n=5)83-101%94-105%78-89%97-104%
Heparin Plasma (n=5)99-105%98-105%96-104%85-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Lysozyme (LZM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Lysozyme (LZM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Lysozyme (LZM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Lysozyme (LZM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range12.5-800pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-10192
EDTA Plasma (n=5)91-9894
Heparin Plasma (n=5)95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Lysozyme (LZM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Lysozyme (LZM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LYZ Microplate96 Wells (12 x 8 Well Strips)
LYZ Lyophilized Standard2
100X Biotinylated LYZ Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity4.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysozyme (LZM). No significant cross-reactivity or interference between Lysozyme (LZM) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolLYZ
Gene Full Namelysozyme
Alias Symbols1,4-beta-N-acetylmuramidase C;c-type lysozyme;lysozyme C;lysozyme F1;LYZF1;LZM.
NCBI Gene Id4069
Protein NameLysozyme C
Description of TargetLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Uniprot IDP61626
Protein Accession #NP_000230.1
Nucleotide Accession #NM_000239.2