MCM7 (full length) Mouse Monoclonal Antibody [Clone ID: 4B4]
Specifications
Product Data | |
Clone Name | 4B4 |
Applications | IF, WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. Immunocytochemistry: 5 µg/ml. |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant full-length human MCM7 |
Formulation | PBS (pH 7.2)/1% sucrose. No preservative is added. State: Azide Free State: Lyophilized Ig fration |
Reconstitution Method | Restore with 100 µl distilled water. |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Prior to reconstitution store at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | The replication of the large genome of eukaryotic cells is made possible by the use of multiple replication origins per chromosome. However, multiple origins must be strictly regulated if all chromosomal sequences are to be replicated only once in S phase, and re-replication of DNA before completing mitosis is prohibited. This characteristic is thought to be due to the properties of “initiation” proteins, such as Orc (Origin recognition complex), Cdc6 and MCM (Minichromosome maintenance) protein family. Experiments used with yeast and Xenopus had clearly s hown that the replication preinitiation complex is assembled in an ordered sequence as Orc recruits Cdc6, which in turn recruits MCM complex (consisting of MCM 2-7 proteins). MCM proteins occur in high copy numbers in nuclei of human cells. They appear to be either free in the nucleoplasm or bound to chromatin. The fraction of structure-bound mammalian MCM proteins is highest at the beginning of S phase, but gradually decreases during progression of replication. |
Synonyms | CDC47, MCM2, CDC47 homolog, P1.1-MCM3 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunocytochemistry |
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