Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder)

Cat# D9821-25L

Size : 25L

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Grade
Cell Culture Grade

Shipping Temp
RT

Storage Temp
RT/4°C

Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.

Also Available:
D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)
D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)
D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)
D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)
D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)

Appearance: White, homogeneous, free flowing powder

Solubility (1X): Colorless, clear, complete.
Some precipitate may be observed as a 10X due to excess concentration of salts. Sterile filter after solubilization.

pH: As Reported

Endotoxin: ≤1EU/ml

Formulation:
Components g/L
Calcium Chloride•2H2O 1.325
Magnesium Chloride•6H2O 1.00
Potassium Chloride 2.00
Potassium Phosphate Mono 2.00
Sodium Chloride 80.00
Sodium Phosphate Dibasic 11.50
Total: 97.8g/L

Directions:
To make a 10X solution:
Dissolve 97.8g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers..

Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

USB recommends the preparation of this product as a 1X solution.

To make a 1X solution:
Dissolve 9.78g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.