CD45RA antibody [HI100]
Cat# GTX01464-100ug
Size : 100ug
Brand : Genetex
Contact local distributor :
Phone : +1 850 650 7790
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | HI100 |
Isotype | IgG2b |
Application | WB, IHC-P, IHC-Fr, FACS, Neutralizing/Inhibition |
Reactivity | Human, Chimpanzee |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX01464 on CiteAb
PRODUCT
Summary
The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others. The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | Assay dependent |
| IHC-P | Assay dependent |
| IHC-Fr | Assay dependent |
| FACS | Assay dependent |
| Neutralizing/Inhibition | Assay dependent |
Not tested in other applications.
Calculated MW
147 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Purity
> 90% (determined by SDS-PAGE)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , LCA , LY5 , PTPRC , T200 , protein tyrosine phosphatase, receptor type C , CD45.1 , CD45.2 , CD45RA , CD45RB , CD45RC
Cellular Localization
Membrane,Membrane raft
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Clone Reference
2012; Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation.
Application : FACS
Reactivity : Human
2011; Expansion of a unique CD57?NKG2Chi natural killer cell subset during acute human cytomegalovirus infection.
Application : FACS
Reactivity : Human
2009; Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa.
Application : IHC-P
Reactivity : Human
2006; Anti-CD45RO suppresses human immunodeficiency virus type 1 replication in microglia: role of Hck tyrosine kinase and implications for AIDS dementia.
Application : WB
Reactivity : Human
2003; Naive T cell recruitment to nonlymphoid tissues: a role for endothelium-expressed CC chemokine ligand 21 in autoimmune disease and lymphoid neogenesis.
Submit a Reference Application : IHC-Fr
Reactivity : Human
REVIEW
Clone Reference
2012; Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation.
Application : FACS
Reactivity : Human
2011; Expansion of a unique CD57?NKG2Chi natural killer cell subset during acute human cytomegalovirus infection.
Application : FACS
Reactivity : Human
2009; Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa.
Application : IHC-P
Reactivity : Human
2006; Anti-CD45RO suppresses human immunodeficiency virus type 1 replication in microglia: role of Hck tyrosine kinase and implications for AIDS dementia.
Application : WB
Reactivity : Human
2003; Naive T cell recruitment to nonlymphoid tissues: a role for endothelium-expressed CC chemokine ligand 21 in autoimmune disease and lymphoid neogenesis.
Application : IHC-Fr
Reactivity : Human
2002; CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase.
Application : Neutralizing/Inhibition
Reactivity : Human
| SDS | |
|---|---|
| Sodium Azide.pdf | |