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> Thrombospondin antibody [A6-1]

Thrombospondin antibody [A6-1]

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Cat# GTX21823-250ul

Size : 250ul

Brand : Genetex

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Phone : +1 850 650 7790

Anti-Thrombospondin antibody [A6-1] used in IHC (Paraffin sections) (IHC-P). GTX21823
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A6-1

Isotype

 IgG1

Application

 WB, IHC-P, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
250 μl
Datasheet File
View product citations for antibody GTX21823 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:25‐1:50
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Product Note

This antibody is specific to 450 kDa (non-reduced) and 170 to 180 kD (reduced) form of thrombospondin.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_384698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 1 , THBS , THBS-1 , TSP , TSP-1 , TSP1

Cellular Localization

Secreted , Endoplasmic reticulum

Background

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-Thrombospondin antibody [A6-1] used in IHC (Paraffin sections) (IHC-P). GTX21823

GTX21823 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX21823 Thrombospondin antibody [A6.1].

Application Reference

X Wang et al. Adv Sci (Weinh) 2023; : e2301428 Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage
Application : Neutralizing/Inhibition
Reactivity : Mouse
Chu YY et al. Mol Neurobiol 2015; (Epub) Astrocytic CCAAT/Enhancer Binding Protein δ Regulates Neuronal Viability and Spatial Learning Ability via miR-135a.
Application : WB
Reactivity : Human

Clone Reference

Dixit VM et al. J Biol Chem. 1986; 261 (4):22859 Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes.
Application : WB, Neutralizing/Inhibition

REVIEW

Thrombospondin antibody [A6-1] Cat. No. GTX21823

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Thrombospondin antibody [A6-1] used in Western Blot (WB). GTX21823
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Mol Neurobiol in 2016. PMID: 26208701
Anti-Thrombospondin antibody [A6-1] used in Western Blot (WB). GTX21823
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mol Neurobiol in 2016. PMID: 26208701
Anti-Thrombospondin antibody [A6-1] used in Western Blot (WB). GTX21823
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Neurobiol in 2016. PMID: 26208701
Application Reference
X Wang et al. Adv Sci (Weinh) 2023;:e2301428 Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage
Application : Neutralizing/Inhibition
Reactivity : Mouse
Chu YY et al. Mol Neurobiol 2015; (Epub) Astrocytic CCAAT/Enhancer Binding Protein δ Regulates Neuronal Viability and Spatial Learning Ability via miR-135a.
Application : WB
Reactivity : Human
Clone Reference
Dixit VM et al. J Biol Chem. 1986; 261 (4):22859 Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes.
Application : WB, Neutralizing/Inhibition
SDS
PBS.pdf
Sodium Azide.pdf