26S Proteasome p50 (112) AC
Cat# sc-65745 AC
Size : 500µg/ml,25%ag
Brand : Santa Cruz Biotechnology
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Anticorps 26S Proteasome p50 (112): sc-65745
- L'anticorps 26S Proteasome p50 112 est un monoclonal IgG2b L'Anticorps 26S Proteasome p50, cité dans 2 publications, fourni en 200 µg/ml
- raised against 26S Proteasome purified from embryos of Drosophila melanogaster origin
- L'Anticorps 26S Proteasome p50 (112) est recommandé pour la détection de p50 subunit of the 19S regulatory base complex of the 26S Proteasome d'origine d par WB et IP
- Anti-L'Anticorps 26S Proteasome p50 (112) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for 26S Proteasome p50 Antibody (112). This work is in progress.
ACCÈS RAPIDE AUX LIENS
, 26S Proteasome p39A, 26S Proteasome p42A, 26S Proteasome p42C, 26S Proteasome p42D, 26S Proteasome p42E, 26S Proteasome p48A, 26S Proteasome p50, 26S Proteasome p54 et 26S Proteasome p58
26S Proteasome p50 Antibody (112) is an IgG2b mouse monoclonal 26S Proteasome p50 antibody (also designated 26S Proteasome p50 antibody) that detects the 26S Proteasome p50 protein of Drosophila melanogaster origin by WB and IP. 26S Proteasome p50 Antibody (112) is available as both the non-conjugated anti-26S Proteasome p50 antibody form, as well as multiple conjugated forms of anti-26S Proteasome p50 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The 26S proteasome is a large complex involved in the intracellular degradation of proteins in eukaryotes. Ubiquitination by E3 ubiquitin ligases targets proteins for degradation by this complex. The 26S proteasome plays an important role in the regulation of many biological processes. It is composed of over 30 different subunits and contains at least two copies of each subunit. Assembly of this large complex is ATP-dependent. Due to its size, it is fairly unstable and often disassociates into subcomplexes (including a 20S core and two 19S regulatory complexes). The 26S proteasome p50 (also known as Rpt5 in yeast and S6′ in human) is one of the six ATPase base subunits of the 19S regulatory complex. The 19S regulatory complex recognizes ubiquitinated proteins, removes the ubiquitin chains and translocates the proteins to the 20S core for degradation. The 26S proteasome p50 is a polyubiquitin interacting protein that plays a role in proteolytic signal recognition.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. | Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
- A proteasomal ATPase subunit recognizes the polyubiquitin degradation signal. | Lam, YA., et al. 2002. Nature. 416: 763-7. PMID: 11961560
- The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems. | Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
- ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle. | Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
- The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. | Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
- The proteasome restricts permissive transcription at tissue-specific gene loci in embryonic stem cells. | Szutorisz, H., et al. 2006. Cell. 127: 1375-88. PMID: 17190601
- Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. | Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
- Aging perturbs 26S proteasome assembly in Drosophila melanogaster. | Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
- Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. | Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-26S Proteasome p50 Antibody (112) has 2 citations in a variety of scientific publications.
Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
What is the recommended starting dilution for Western blot for 26S Proteasome p50 (112): sc-65745 monoclonal antibody?
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