Viruses are purified over 2 rounds of CsCl gradients and then dialyzed and formulated in A195 buffer.
1 to 1.3 e12 particles/mL
none
Ad5 E1 / E3 deleted
none
none
Store virus at -80 °C and avoid freeze-thaw cycles. Only make aliquots in the original buffer at the original concentration. Aliquots should be no less than 50 µL and stored in a screw cap tube.
Liquid
Viruses are purified over 2 rounds of CsCl gradients and then dialyzed and formulated in A195 buffer.
A195 buffer consisting of 10 mM Tris, 75 mM NaCl, 5% Sucrose (w/v), 0.020% PS-80 (w/v), 1 mM MgCl2, 0.1 mM EDTA, 0.5% EtOH (v/v), 10 mM Histidine, pH at 25°C = 7.4
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, viewable on our website.
