ADAM17 ELISA Kit (Mouse)
Cat# OKCA02169
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
| ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Adam17 ELISA Kit (Mouse) (OKCA02169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ADAM17 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 15.6 pg/mL-1,000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
| ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay Variation <10% CV (n=20) Mean Inter-Assay Variation <12% CV (n=8) | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma And Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 3.9 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | ADAM17 |
---|---|
Alias Symbols | Tac, Tace, CD156b |
NCBI Gene Id | 11491 |
Protein Name | Disintegrin and metalloproteinase domain-containing protein 17 |
Description of Target | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2. |
Uniprot ID | Q9Z0F8 |
Protein Accession # | NP_001264195 |
Nucleotide Accession # | NM_001277266 |