Advanced Oxidation Protein Products (AOPP) BioAssay™ ELISA Kit (Rat)
Cat# 516963-96T
Size : 96Tests
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°CThe Advanced Oxidation Protein Products (AOPP) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AOPP in rat serum, plasma and other biological fluids.
Detection Range:
2.47-200ng/ml
Sensitivity:
0.91ng/ml
Intra-Assay CV:
<10%
Inter-Assay CV:
<12%
Kit Components:
*516963A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use.
*516963B: Standard, 2x1vial
516963C: Standard Diluent, 1x20ml
*516963D: Detection Reagent A (green), 1x1vial
*516963E: Detection Reagent B (red), 1x120ul
516963F: Assay Diluent A, 1x12ml
516963G: Assay Diluent B, 1x12ml
516963H: TMB Substrate, 1x9ml
*516963J: Positive Control, 62.4ng/ml, 1x1vial (Lyophilized)
516963K: Stop Solution, 1x6ml
516963L: Wash Buffer, 30X, 1x20ml
516963M: Reagent Diluent, 1x300ul
*516963B: Standard, 2x1vial
516963C: Standard Diluent, 1x20ml
*516963D: Detection Reagent A (green), 1x1vial
*516963E: Detection Reagent B (red), 1x120ul
516963F: Assay Diluent A, 1x12ml
516963G: Assay Diluent B, 1x12ml
516963H: TMB Substrate, 1x9ml
*516963J: Positive Control, 62.4ng/ml, 1x1vial (Lyophilized)
516963K: Stop Solution, 1x6ml
516963L: Wash Buffer, 30X, 1x20ml
516963M: Reagent Diluent, 1x300ul
Precaution:
The Stop Solution (516963K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:
Store *516963A, *516963B, *516963D, *516963E and *516963J at -20°C. After reconstitution of *516963J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 516963J: Positive Control
Intended Use:
The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:
The positive control vial contains rat AOPP in rat serum (lyophilized powder). Note: The rat based serum used in this product are from healthy rat of SPF standard (specific pathogen-free animals).
Expected Values:
62.4ng/ml
Usage:
Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.