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> Anti-Pepsin (HRP) Polyclonal Antibody

Anti-Pepsin (HRP) Polyclonal Antibody

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Cat# P3270-15-100ug

Size : 100ug

Brand : US Biological

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Phone : +1 850 650 7790

P3270-15 Pepsin (HRP)

Clone Type
Polyclonal
Host
goat
Source
porcine
Swiss Prot
P00791
Isotype
IgG
Grade
Highly Purified
Applications
DB E IHC WB
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1;2000-1:10,000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:250-1:1250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: porcine|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 100ul of sterile dH2O. Labeled with Horseradish peroxidase (HRP).|Purity: Purified by delipidation, salt fractionation and ion exchange chromatography. |Immunogen: Pepsin (Porcine Stomach).|Specificity: Recognizes porcine pepsin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxide, anti-goat serum and purified and partially purified pepsin (Porcine Stomach)||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Pepsin (Porcine Stomach).
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 100ul of sterile dH2O. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Recognizes porcine pepsin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxide, anti-goat serum and purified and partially purified pepsin (Porcine Stomach)
References
1. Farr & Nakane, J Immunol. Methods, 47:129-144, 1981.