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> Anti-Rat IgA (alpha chain) (Biotin Conjugated) Secondary Antibody

Anti-Rat IgA (alpha chain) (Biotin Conjugated) Secondary Antibody

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Cat# ASR2826

Size : 1mg

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • WB - Anti-Rat IgA (alpha chain) (Biotin Conjugated) Secondary Antibody ASR2826
    Western Blot of Biotin Conjugated Goat Anti-Rat IgA (Alpha chain) Secondary Antibody. Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None.
Product Information
Description Anti-RAT IgA (alpha chain) (GOAT) Antibody Biotin Conjugated
Host Goat
Conjugate Biotin
Target Species Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgA
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgA alpha heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum.  No reaction was observed against other Rat heavy or light chain proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.