Reagents and instruments for immunology, cell biology and molecular biology.

Atropine [51-55-8]

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Cat# HY-B1205-100mg

Size : 100mg

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Phone : +1 850 650 7790

Brand : MedChemExpress

Contact local distributor :

Phone : +1 850 650 7790

Atropine (Tropine tropate) is a competitive muscarinic acetylcholine receptor (mAChR) antagonist with IC₅₀ values of 0.39 and 0.71 nM for Human mAChR M₄ and Chicken mAChR M₄, respectively. Atropine inhibits ACh-induced relaxations in human pulmonary veins. Atropine can be used for research of anti-myopia and bradycardia.

For research use only. We do not sell to patients.

Atropine Chemical Structure

CAS No. : 51-55-8

This product is a controlled substance and not for sale in your territory.

Based on 14 publication(s) in Google Scholar

Other Forms of Atropine:

Atropine sulfate monohydrate In-stock
Atropine sulfate In-stock
(Rac)-Atropine-d₃ In-stock
Atropine hydrobromide Get quote

Description

IC₅₀ & Target

mAChR4

In Vitro

Atropine (Tropine tropate; 1 μM; pulmonary veins and arteries) inhibits ACh-induced relaxations in human pulmonary veins^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Atropine (Tropine tropate; 10 mg/kg; i.p.; once, for 40 minutes; Peromyscus sp.) inhibits the cardiac arrhythmia which normally occurs throughout torpor^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: White-footed mice (Peromyscus sp.)^[2]

Dosage: 10 mg/kg

Administration: Intraperitoneal injection; once, for 40 minutes

Result: Increased heart rate was a decrease in cardiac arrhythmia.

Clinical Trial

Molecular Weight

289.37

Formula

C₁₇H₂₃NO₃

CAS No.

51-55-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@@H]1C[C@@H](N2C)CC[C@@H]2C1)C(CO)C3=CC=CC=C3

Structure Classification

Alkaloids
Other Alkaloids

Initial Source

Plants
Solanaceae
Atropa belladonna Linn.

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 96.6 mg/mL (333.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.9 mg/mL (10.02 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4558 mL	17.2789 mL	34.5578 mL
5 mM	0.6912 mL	3.4558 mL	6.9116 mL
10 mM	0.3456 mL	1.7279 mL	3.4558 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (289 KB) SDS (0 KB)

COA (251 KB) HNMR (252 KB) LCMS (85 KB)

Handling Instructions (2659 KB)

References

[1]. McBrien NA, et, al. How does atropine exert its anti-myopia effects? Ophthalmic Physiol Opt. 2013 May;33(3):373-8. [Content Brief]

[2]. Morhardt JE. Heart rates, breathing rates and the effects of atropine and acetylcholine on white-footed mice (Peromyscus sp.) during daily torpor. Comp Biochem Physiol. 1970 Mar 15;33(2):441-57. [Content Brief]

[3]. Carr BJ, et, al. Myopia-Inhibiting Concentrations of Muscarinic Receptor Antagonists Block Activation of Alpha2A-Adrenoceptors In Vitro. Invest Ophthalmol Vis Sci. 2018 Jun 1;59(7):2778-2791. [Content Brief]

[4]. Walch L, et, al. Evidence for a M(1) muscarinic receptor on the endothelium of human pulmonary veins. Br J Pharmacol. 2000 May;130(1):73-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.4558 mL	17.2789 mL	34.5578 mL	86.3946 mL
	5 mM	0.6912 mL	3.4558 mL	6.9116 mL	17.2789 mL
	10 mM	0.3456 mL	1.7279 mL	3.4558 mL	8.6395 mL
DMSO	15 mM	0.2304 mL	1.1519 mL	2.3039 mL	5.7596 mL
	20 mM	0.1728 mL	0.8639 mL	1.7279 mL	4.3197 mL
	25 mM	0.1382 mL	0.6912 mL	1.3823 mL	3.4558 mL
	30 mM	0.1152 mL	0.5760 mL	1.1519 mL	2.8798 mL
	40 mM	0.0864 mL	0.4320 mL	0.8639 mL	2.1599 mL
	50 mM	0.0691 mL	0.3456 mL	0.6912 mL	1.7279 mL
	60 mM	0.0576 mL	0.2880 mL	0.5760 mL	1.4399 mL
	80 mM	0.0432 mL	0.2160 mL	0.4320 mL	1.0799 mL
	100 mM	0.0346 mL	0.1728 mL	0.3456 mL	0.8639 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Atropine [51-55-8]

Cat# HY-B1205-100mg

Contact local distributor :

Brand : MedChemExpress

Contact local distributor :

Other Forms of Atropine:

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Atropine Related Classifications

Keywords:

You might also be interested by the following products:

Atropine [51-55-8]

Cat# HY-B1205-100mg

Contact local distributor :

Brand : MedChemExpress

Contact local distributor :

Other Forms of Atropine:

Atropine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Atropine Related Classifications

Keywords:

You might also be interested by the following products: