Bauhinia purpurea Lectin (BPL/BPA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.

Source:
Camel's foot tree

Purity:
Affinity purified

Appearance:
Light orange, clear liquid

Form:
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Concentration:
≥2mg/ml

Blood Specificity:
A, B, O (-SA)

Sugar Specificity:
Galbeta; 3GalNAc

Inhibitory Carbohydrate:
Lactose

Fluorescence:
Green

Divalent Ions:
None Required

Excitation:
495-500nm Maximum

Emission:
514-521nm Maximum

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.