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> BNIP3 Antibody

BNIP3 Antibody

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Cat# ASM10545-A680-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - BNIP3 Antibody ASM10545
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Tissue: Fibroblast cell line (NIH 3T3). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.
  • WB - BNIP3 Antibody ASM10545
    Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .
  • WB - BNIP3 Antibody ASM10545
    Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q12983
Other Accession NP_004043.3
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human BNIP3 Polyclonal
Target/Specificity Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
Other Names NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody
Immunogen Synthetic peptide from the C-terminal of human BNIP3
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion | Mitochondrion outer membrane