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> Calcineurin A Antibody

Calcineurin A Antibody

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Cat# ASM10431-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Calcineurin A Antibody ASM10431
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite.
  • IHC - Calcineurin A Antibody ASM10431
    Immunohistochemistry analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: General epidermis.
  • WB - Calcineurin A Antibody ASM10431
    Western blot analysis of Rat Brain cell lysates showing detection of ~61 kDa Calcineurin A protein using Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~61 kDa.
  • ICC/IF - Calcineurin A Antibody ASM10431
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (ASM10431) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Primary Accession Q08209
Other Accession Q08209
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Dog
Clonality Polyclonal
Description Rabbit Anti-Human Calcineurin A Polyclonal
Target/Specificity Detects ~61kDa.
Other Names Alpha isoform formerly PPP2B Antibody, Calcineurin A alpha Antibody, Calcineurin A1 Antibody, CalcineurinA Antibody, Calmodulin dependent calcineurin A subunit alpha isoform Antibody, Calmodulin-dependent calcineurin A subunit alpha isoform Antibody, CALN Antibody, CALNA Antibody, CALNA 1 Antibody, CAM PRP catalytic subunit Antibody, CCN 1 Antibody, Protein phosphatase 2B catalytic subunitProtein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform Antibody, Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA Antibody, Protein phosphatase 3 catalytic subunit alpha isozyme Antibody, Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform Antibody
Immunogen Human Calcineurin A peptide (AA 264-283)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-175 was sufficient for detection of Calcineurin A in 20 µg of Heat shock Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus