Caspase 10 (CASP10) (full length) Mouse Monoclonal Antibody [Clone ID: 4C1]
CAT#: AM26608AF-N
Caspase 10 (CASP10) (full length) mouse monoclonal antibody, clone 4C1, Azide Free
Specifications
Product Data | |
Clone Name | 4C1 |
Applications | WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. For details see protocol below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant full-length of human FLICE2 |
Specificity | This antibody detects specifically 57 and 58 kDa of human caspase-10, 43 kDa and 30 kDa of human active caspase-10 on Western blotting (Ref.8). This antibody reacts with isoforms caspase-10/a, 10/b and 10/d (Ref.17). |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Database Link | |
Background | Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathway that leads to the recruitment and activation of caspases, a family of cysteine-containing , aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Ca spase-10 (also known as Mch4, FLICE2 and ICE-LAP4) is a ~58 kDa protein. This caspase acts upstream of the apoptosis induced cascade. Once this caspase is activated by certain apoptotic stimuli, this protein may be responsible for the activation of the other caspases such as caspase-3, -4, -7, -8, and -9. This antibody was made against human-originated immunogen, and detects human caspase-10 specifically. |
Synonyms | CASP-10, CASP10, MCH4, FLICE2 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Apoptosis induction |
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