CD45R (B220, TXRD, Ly-5, Lyt-4) (PE/Texas Red)

CD45R, also known as B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and prior to the availability of anti-CD19 monoclonal antibodies was commonly used as a pan B-cell marker. It also reacts with certain activated T cells as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells. In vivo administration has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.

Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 0.2ug/10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE and Texas Red conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Mab

IgG2a

RA3-6B2

rat

mouse

~0.1mg/ml

Supplied as a liquid in PBS, sodium azide. Labeled with PE/Texas Red.

Purified

Abelson murine leukemia virus-induced pre-B cell lymphoma.

Recognizes mouse CD45R. Species Crossreactivity

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.