CHI3L1 ELISA Kit (Human)
Cat# OKEH02691
Size : 96Wells
Brand : Aviva Systems Biology
CHI3L1 ELISA Kit (Human) : 96 Wells (OKEH02691)
| Datasheets/Manuals | Printable datasheet for CHI3L1 ELISA Kit (Human) : 96 Wells (OKEH02691) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CHI3L1 ELISA Kit (Human) (OKEH02691) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human CHI3L1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CHI3L1 and incubated. The CHI3L1 found in the sample or standards competes with the biotinylated CHI3L1 for limited binding sites on the immobilized anti-Human CHI3L1 antibody. Excess unbound biotinylated CHI3L1 and sample or standard CHI3L1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CHI3L1 captured in the well and inversely proportional to the amount of CHI3L1 which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.2% (n = 20) Mean Inter-assay CV%: < 9.6% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 12 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Chitinase-3-like protein 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | CHI3L1 |
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| Gene Full Name | chitinase 3 like 1 |
| Alias Symbols | 39 kDa synovial protein, ASRT7, cartilage glycoprotein 39, CGP-39, chitinase 3-like 1 (cartilage glycoprotein-39), chitinase-3-like protein 1, GP-39, GP39, HC-gp39, hCGP-39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40. |
| NCBI Gene Id | 1116 |
| Protein Name | Chitinase-3-like protein 1 |
| Description of Target | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. |
| Uniprot ID | P36222 |
| Protein Accession # | NP_001267.2 |
| Nucleotide Accession # | NM_001276.2 |
| Protein Size (# AA) | 383 |
| Molecular Weight | 43 kDa |