Concanavalin A (Jackbean) Lectin (Con A) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.

Source:
Jackbean

Purity:
Affinity purified

Appearance:
Light orange, clear liquid

Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Concentration:
≥2mg/ml

Blood Specificity:
Relatively weak blood agglutinin

Sugar Specificity:
alpha-Mannose, alpha-Glucose

Inhibitory Carbohydrate:
alpha-Methylmannoside + alpha-Methylglucoside

Fluorescence:
Green

Divalent Ions:
Ca++, Mn++

Excitation:
495-500nm Maximumm

Emission:
514-521nm Maximum

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.