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> COVID-19 Spike-ACE2 Binding Assay Kit

COVID-19 Spike-ACE2 Binding Assay Kit

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Cat# CoV-ACE2S2-1

Size : 1PlateKit

Brand : RayBiotech


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Figure adapted from Phan et al., Biomacromolecules, 2023.The kit was used to measure concentration-dependent reduction of recombinant SARS-CoV-2 spike RBD binding to human ACE2 in an ELISA competition assay. Figure adapted from Beeg et al., International Journal of Molecular Sciences, 2023.Figure adapted from Amruta et al., PLOS ONE, 2023.The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike receptor-binding domain (RBD) interaction with human ACE2 in the presence of bazedoxifene. Figure adapted from Omotuyi et al., Microbial Pathogenesis, 2023.The kit was used to assess changes in SARS-CoV-2 spike receptor-binding domain interaction with human ACE2 following visible-light photocatalytic treatment. Figure adapted from Matsuyama et al., Biotechnology Letters, 2023.COVID-19 Spike-ACE2 Binding Assay Kit

Figure adapted from Phan et al., Biomacromolecules, 2023.

COVID-19 Spike-ACE2 Binding Assay Kit

The RayBio® COVID-19 Spike-ACE2 binding assay kit is a patented, rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.

Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman, SARS-CoV-2
Compatible Sample TypesPlasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number
QHD43416
Gene Symbols
ACE2, S, RBD, SRBD, S-RBD
Protein Name / Synonyms
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Format
ACE2 on plate + Spike RBD query
Quantitative/Semi-QuantitativeSemi-Quantitative
Solid Support
96-well Microplate
Method Of DetectionColorimetric
Research AreaProtein Therapeutics, Receptor-Ligand Binding, Infectious Disease, COVID-19
Shipping TypeBlue ice
Storage4°C
Note
The binding assay comes in two different options based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study.

Product Features

  • Evaluate inhibitor activity
  • Screen inhibitors of the SARS-CoV-2 Spike-ACE2 complex
  • Characterize antibody function
  • Vaccine development
  • Selection of patient serum for antibody therapy

Validation Data

Inhibition of Spike-ACE2 by anti-viral compounds
Inhibition of Spike-ACE2 by anti-viral compounds
Four small molecules tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2).
Inhibition of Spike-ACE2 by anti-Spike antibodies
Inhibition of Spike-ACE2 by anti-Spike antibodies
Four antibodies tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2). nAb 1 = 130-10807; nAb 2 = 130-10844; nAb 3 = 130-10845; negative control = 130-10813.

Typical Data

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of recombinant SARS-CoV-2 spike RBD binding to hACE2, showing dose-dependent suppression of the spike–ACE2 interaction by biomimetic spike protein nanoparticles. Figure adapted from Phan et al., Biomacromolecules, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure concentration-dependent reduction of recombinant SARS-CoV-2 spike RBD binding to human ACE2 in an ELISA competition assay. Figure adapted from Beeg et al., International Journal of Molecular Sciences, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike RBD binding to human ACE2 following acetic acid treatment, demonstrating reduced spike–ACE2 interaction after viral protein exposure to acetic acid. Figure adapted from Amruta et al., PLOS ONE, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike receptor-binding domain (RBD) interaction with human ACE2 in the presence of bazedoxifene. Figure adapted from Omotuyi et al., Microbial Pathogenesis, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to assess changes in SARS-CoV-2 spike receptor-binding domain interaction with human ACE2 following visible-light photocatalytic treatment. Figure adapted from Matsuyama et al., Biotechnology Letters, 2023.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. The life of the kit can be extended up to 6 months from the date of shipment by storing the kit reagents (Item F, C, and D) at -20°C. All other components should be stored at 4°C.

citations

  • Nanostructured biohybrid material with wide-ranging antiviral action

    Year:
    2023
    Journal Name:
    Nano Research

  • Biomimetic SARS-CoV-2 Spike Protein Nanoparticles

    Year:
    2023
    Journal Name:
    Biomacromolecules
    PubMed ID:
    37001147

  • A Comprehensive Technology Platform for the Rapid Discovery of Peptide Inhibitors against SARS-CoV-2 Pseudovirus Infection

    Year:
    2023
    Journal Name:
    International Journal of Molecular Sciences

  • Effects of spike proteins on angiotensin converting enzyme 2 (ACE2)

    Species:
    Human
    Sample Type:
    HEK293 cell
    Year:
    2023
    Journal Name:
    Archives of Biochemistry and Biophysics

  • Effect of acetic acid inactivation of SARS-CoV-2

    Species:
    Monkey
    Sample Type:
    VeroE6 cell
    Year:
    2023
    Journal Name:
    Plos One

  • Benzimidazole compound abrogates SARS-COV-2 receptor-binding domain (RBD)/ACE2 interaction In vitro

    Year:
    2023
    Journal Name:
    Microbial Pathogenesis

  • Bridelia ferruginea phytocompounds interact with SARS-COV-2 drug targets: Experimental validation of corilagin contribution

    Year:
    2023
    Journal Name:
    Scientific African

  • Inactivation of SARS-CoV-2 variants by nitrogen-doped titanium dioxide loaded with metals as visible-light photocatalysts

    Year:
    2023
    Journal Name:
    Biotechnology Letters

  • Purification and characterisation of heparin-like sulfated polysaccharides with potent anti-SARS-CoV-2 activity from snail mucus of Achatina fulica

    Species:
    Large snail
    Sample Type:
    mucus
    Year:
    2023
    Journal Name:
    Carbohydrate Research

  • D614G SARS-CoV-2 Pseudovirus Infectivity and Binding of Spike Protein to the ACE2 Receptor Inversely Correlates with Serum SARS-CoV-2-Specific IgG Levels

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Viral Immunology

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