CPE (Carboxypeptidase E, CPH, Enkephalin Convertase, Carboxypeptidase H, Prohormone-processing carboxypeptidase)

Cat# 362661-200ul

Size : 200ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Host
rabbit

Isotype
IgG

Grade
Affinity Purified

Applications
E IC IHC WB

Shipping Temp
Blue Ice

Storage Temp
-20°C

Carboxypeptidase E cleaves C-terminal aa residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E is found in neuroendocrine cells and in adrenal gland chromaffin cells. The glycoprotein can be both membrane-associated or soluble. At the C-end of the molecule lies an amphiphilic alpha-helix which might be responsible for the membrane localisation. Carboxypeptidase E is found in all species of vertebrates that have been examined, and is also present in many other organisms that have been studied. Interesting, carboxypeptidase E is not found in the fruit fly, and another enzyme fills in for carboxypeptidase E in this organism.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.

AA Sequence:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV NEKEGGPNNH LLKNMKKIVD QNTKLAPETK AVIHWIMDIP FVLSANLHGG DLVANYPYDE TRSGSAHEYS SSPDDAIFQS LARAYSSFNP AMSDPNRPPC RKNDDDSSFV DGTTNGG

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Type
Pab

Isotype
IgG

Host
rabbit

Source
human

Concentration
~0.5mg/ml

Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Purity
Purified by immunoaffinity chromatography.

Immunogen
Recombinant protein corresponding to Ile151-Gly317 of human CPE expressed in E. coli.

Specificity
Recognizes human CPE.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.