CPE (Carboxypeptidase E, CPH, Enkephalin Convertase, Carboxypeptidase H, Prohormone-processing carboxypeptidase)
Cat# 362661-200ul
Size : 200ul
Brand : US Biological
Host
rabbitIsotype
IgGGrade
Affinity PurifiedApplications
E IC IHC WBShipping Temp
Blue IceStorage Temp
-20°CCarboxypeptidase E cleaves C-terminal aa residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E is found in neuroendocrine cells and in adrenal gland chromaffin cells. The glycoprotein can be both membrane-associated or soluble. At the C-end of the molecule lies an amphiphilic alpha-helix which might be responsible for the membrane localisation. Carboxypeptidase E is found in all species of vertebrates that have been examined, and is also present in many other organisms that have been studied. Interesting, carboxypeptidase E is not found in the fruit fly, and another enzyme fills in for carboxypeptidase E in this organism.
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.
AA Sequence:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV NEKEGGPNNH LLKNMKKIVD QNTKLAPETK AVIHWIMDIP FVLSANLHGG DLVANYPYDE TRSGSAHEYS SSPDDAIFQS LARAYSSFNP AMSDPNRPPC RKNDDDSSFV DGTTNGG
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Pab
Isotype
IgG
Host
rabbit
Source
human
Concentration
~0.5mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Immunogen
Recombinant protein corresponding to Ile151-Gly317 of human CPE expressed in E. coli.
Specificity
Recognizes human CPE.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.