CPLX1
Cat# 521796-100ul
Size : 100ul
Brand : US Biological
Host
rabbitIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBShipping Temp
Blue IceStorage Temp
-20°C921-S, complexin 1, Complexin I, Complexin-1, CPLX1, CPLX1_HUMAN, CPX I, CPX-I, CPX1, Synaphin 2, Synaphin-2
Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed:21785414). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed:21785414). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight.
Immunohistochemistry: 1:50-1:200
ELISA (peptide): 1:20,000-1:40,000
Optimal dilutions to be determined by the researcher.
Immunohistochemistry: 1:50-1:200
ELISA (peptide): 1:20,000-1:40,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Pab
Isotype
IgG
Host
rabbit
Source
human
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Immunogen
Synthetic peptide corresponding to human CPLX1. Species sequence homology
Specificity
Recognizes endogenous levels of human CPLX1. Species Crossreactivity
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.