CSF1 Protein Lysate

Cat# OCOA03207

Size : 20ug

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for CSF1 Protein Lysate (OCOA03207)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCSF1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCSF1
Gene Full Namecolony stimulating factor 1
Alias Symbolscolony stimulating factor 1 (macrophage);CSF-1;lanimostim;macrophage colony stimulating factor 1;macrophage colony-stimulating factor 1;MCSF.
NCBI Gene Id1435
Protein NameNM_172210, NP_757349, P09603
Description of TargetHomo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA, Macrophage colony-stimulating factor 1, macrophage colony-stimulating factor 1 isoform b precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P09603
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_757349
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_172210
Molecular Weight47.7 kDa