DRD4 (D(4) Dopamine Receptor, D(2C) Dopamine Receptor, Dopamine D4 Receptor) (FITC)

Cat# 029457-FITC-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Host
mouse

Conjugate
FITC

Isotype
IgG1,k

Clone Number
2B9

Grade
Affinity Purified

Applications
IC WB

Shipping Temp
Blue Ice

Storage Temp
-20°C

Five subtypes of dopamine receptors exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). As members of the G protein-coupled receptor (GPCR) family, dopamine receptors initiate their biological function by coupling to GTP regulatory proteins (G proteins). D1-like receptors, DRD1 and DRD5, stimulate adenylyl cyclase whereas D2-like receptors, DRD2, DRD3 and DRD4, are coupled to inhibitory G proteins and their stimulation leads to the inhibition of adenylyl cyclase activity, and subsequent respective cellular signaling. All five subtypes have seven transmembrane domains, an extracellular amino terminus, and an intracellular carboxyl terminus. Allelic variants of the D4 dopamine receptor (DRD4) are known characterized risk factors of ADHD. It is reported that hypoxia enhances DRD4 promoter activity by several fold. It is also thought that dopamine receptors such as DRD4 are involved in neuroexcitation and in a variety of other neurological and psychiatric disorders.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: detected DRD4 in 10ug of mouse brain tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain tissue lysate

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Product Type
Mab

Isotype
IgG1,k

Clone No
2B9

Host
mouse

Source
human

Concentration
As Reported

Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

Purity
Purified by Protein G affinity chromatography.

Immunogen
Synthetic linear peptide corresponding to human DRD4.

Specificity
Recognizes human DRD4. Species Crossreactivity


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.