ASM10237-A633-100 | GABA-B Receptor 1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone S93A-49 (ASM10237). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (ASM10237) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-B Receptor 1 Antibody (D) Composite.
Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49 (ASM10237). Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody (ASM10237) at 1:1000.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q9Z0U4
Other Accession	NP_112290.2
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat GABA-B Receptor 1 Monoclonal IgG1
Target/Specificity	Detects ~115kDa. No cross-reactivity against GABA(B)R2.
Other Names	GABA-B receptor 1 Antibody, GABA-B-R1 Antibody, GABR1_Human Antibody, Gamma aminobutyric acid receptor 1 Antibody, GB1 Antibody, GPRC3A Antibody
Clone Names	S93A-49
Immunogen	Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-403 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Membrane \| Cell Junction \| Synapse \| Postsynaptic Cell Membrane

GABA-B Receptor 1 Antibody