Orb11604-100ug | GFP antibody Clinisciences

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GFP antibody

Catalog Number	orb11604
Category	Antibodies
Description	Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression
Species/Host	Goat
Clonality	Polyclonal
Tested applications	IF, IHC-Fr, IHC-P, WB
Isotype	IgG
Immunogen	Purified recombinant peptide produced in E. coli
Concentration	2 mg/ml
Dilution range	WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000
Form/Appearance	PBS, 20% glycerol and 0.05% sodium azide
Purity	This antibody is epitope-affinity purified from goat antiserum
Conjugation	Unconjugated
Target	In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins
Storage	Store at -20 C for long-term storage. Store at 2-8 C for up to one month
Buffer/Preservatives	PBS, 20% glycerol and 0.05% sodium azide
Alternative names	Green fluorescent protein antibody
Note	For research use only
Application notes	The antibody solution should be gently mixed before use.
Expiration Date	12 months from date of receipt.

Primary fibroblasts transduced with ad GFP-Ra1a and immunostained with GFP antibody

AtT-20 transfected with GFP-Rab27a and immunostained with GFP antibody

Western blot analysis of HEK293 transfered cell lysates at 50 ug per lane using GFP antibody