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> Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with FITC Antibody

Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with FITC Antibody

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Cat# orb21372-1ml

Size : 1ml

Brand : Biorbyt


Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with FITC Antibody

SKU: orb21372
This product can be custom made

Description

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat IgG, Fc specific. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in sero- diagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Research Area

Cell Biology

Images & Validation

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Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with FITC Antibody
ReactivityRat

Key Properties

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Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
ConjugationFITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Form/AppearanceFITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information