ASM10184-STR-100 | HCN3 Antibody Clinisciences

Immunohistochemistry analysis using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (ASM10184). Tissue: brain tissue. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (ASM10184) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Granular staining of some Purkinje cells. Staining appears to be focal.
Western Blot analysis of Rat brain membrane lysate showing detection of HCN3 protein using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (ASM10184). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (ASM10184) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (ASM10184). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (ASM10184) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, AM
Primary Accession	O88705
Other Accession	NP_032253.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Mouse HCN3 Monoclonal IgG1
Target/Specificity	Detects ~90kDa. No cross-reactivity against other HCNs.
Other Names	KIAA1535 Antibody, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 Antibody, hyperpolarization activated cyclic nucleotide-gated potassium channel 3 Antibody
Clone Names	S141-28
Immunogen	Fusion protein amino acids 660-779 (C terminus) of mouse HCN3
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-306 was sufficient for detection of HCN3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Membrane

HCN3 Antibody