Disease:

Papilloma

Origin:

HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney..

The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.

The cell line appears to be derived from a single cell based on Southern and FISH analysis.

Species:

Human, Homo sapiens

Tissue:

Kidney, cortex/proximal tubule

Morphology:

Epithelial

Properties:

Adherent

Patient:

Male, adult

Medium:

Keratinocyte Growth Medium (serum-free)(C00013-34)

Subculture:

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin/EDTA. Culture at 5% CO2, 37°C. Feed cells once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO