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> HSP90B1 Antibody - DyLight 488 Conjugated

HSP90B1 Antibody - DyLight 488 Conjugated

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Cat# OAED00197

Size : 50ug

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

  • ™ 488 conjugate) Antibody (OAED00197) in Jurkat cells tissue/cell using Flow Cytometry" data-caption="Flow cytometry analysis of 106 Jurkat cells stained using Grp94 mAb (9G10), DyLight 488 Conjugate at a concentration of 50 ug/ml.Jurkat cells
Datasheets/ManualsPrintable datasheet for Anti-Grp94 (9G10) (DyLight™ 488 conjugate) Antibody (OAED00197)OAED00197
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus
ClonalityMonoclonal
Clone9G10
IsotypeIgG2a
HostRat
ApplicationFC
Reconstitution and StorageLong Term Storage : -20C
ImmunogenNative chicken Grp94.
SpecificityRecognizes human, mouse, rat, bovine, dog, chicken, guinea pig, hamster, monkey, pig, rabbit, sheep and Xenopus Grp94. Detects a band of ~94kDa by Western blot.
FormulationLiquid. In PBS, pH 7.2, containing 0.09% sodium azide.
PurityProtein G affinity purified.
Application InfoFlow Cytometry (1:20)
Optimal conditions must be determined individually for each application.
Gene SymbolHSP90B1
Alias SymbolsECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m
NCBI Gene Id7184
Protein NameEndoplasmin
Description of TargetGlucose-regulated protein 94 (Grp94, gp96), an abundant resident endoplasmic reticulum (ER) lumenal stress protein, belongs to the Hsp90 family of molecular chaperones along with cytosolic Hsp90. Grp94 and such other resident soluble proteins of the ER as the Ca 2+ binding protein subfamily (CaBP, CaBPI, CaBP2 and calreticulin) possess the C-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal considered responsible for the retention of these proteins in the pre-Golgi compar™ents. Stress conditions such as glucose starvation and heat shock which promote protein misfolding or unfolding increase Grp94 expression. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Grp94 and Hsp90 share high sequence identity and apparently identical adenosine nucleotide dependent modes of regulation, although previous data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94 remains unclear. Data supports a ligand dependent regulation of Grp94 function, and suggests a model whereby Grp94 function is regulated through a ligand -dependent conversion of Grp94 from an inactive to an active conformation.
Uniprot IDP14625
Protein interactions
Name# of Products
AMFR164
APOB76
ASGR137
BIRC2191
CSNK2A1551CSNK2A2274EGFR647
EIF2AK319
ERBB2167
FANCA156
FANCC81
H2AFX444
HDAC5906
HSP90B1141HSPA956
LRP1227
Mapk13300
MAPK6386
MTTP15
POLR2E47
PSMA3438
RAE189
RNF185110
SIRT358
SMARCA4392SMARCC1127SMARCC285
TERF1223
TERF2313
TG43
TLR173
TLR2123
TLR4158
UBA5109
UBC7030
VWF73
Biological pathways
Name# of Products
Actin rod assembly8
Anti-apoptosis727
Cellular response to ATP8
ER-associated protein catabolic process48
Protein folding257
Protein transport432
Regulation of phosphoprotein phosphatase activity19
Response to hypoxia218
Sequestering of calcium ion13
Biological process
Name# of Products
Actin rod assembly2
Activation of signaling protein activity involved in unfolded protein response51
Anti-apoptosis239
Cellular response to ATP2
Endoplasmic reticulum unfolded protein response72
ER-associated protein catabolic process24
Protein folding164
Protein transport326
Regulation of phosphoprotein phosphatase activity9
Response to hypoxia205
Response to stress160
Sequestering of calcium ion3
Cellular components
Name# of Products
Cytosol1868
Endoplasmic reticulum837
Endoplasmic reticulum lumen87
Endoplasmic reticulum membrane498
Melanosome87
Microsome250
Midbody66
Perinuclear region of cytoplasm371
Plasma membrane part2
Protein function
Name# of Products
ATP binding2814
Calcium ion binding1062
Low-density lipoprotein particle receptor binding58
Nucleotide binding3928
Protein binding12191
Protein phosphatase binding147
RNA binding1153
Unfolded protein binding252
Virion binding27

  1. What is the species homology for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus".

  2. How long will it take to receive "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 week".

  3. What buffer format is "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    This target may also be called "ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m" in publications.

  5. What is the shipping cost for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.

  6. What is the guarantee for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "HSP90B1 Antibody - DyLight 488 Conjugated (OAED00197)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "HSP90B1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "HSP90B1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "HSP90B1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "HSP90B1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "HSP90B1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "HSP90B1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.