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> HSPB2 (MKBP) Antibody

HSPB2 (MKBP) Antibody

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Cat# ASM10454-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - HSPB2 (MKBP) Antibody ASM10454
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSPB2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - HSPB2 (MKBP) Antibody ASM10454
    Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (ASM10454) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q16082
Other Accession NP_001532.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human HSPB2 (MKBP) Polyclonal
Target/Specificity Detects ~27kDa.
Other Names DMPK-binding protein Antibody, heat shock 27kDa protein 2 Antibody, HSP27 Antibody, HSPB2 Antibody, MKBP Antibody, MGC133245 Antibody
Immunogen Synthetic peptide
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.02% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-194 was sufficient for detection of MKBP in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus