Human Angiogenesis Array GS1000

Cat# GSH-ANG-1000-4

Size : 64SampleKit

Brand : RayBiotech


Human Angiogenesis Array GS1000

G-Series Human Angiogenesis Array 1000 Kit. A combination of 2 non-overlapping arrays to measure the relative expression levels of 60 human angiogenic proteins. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected60
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaAngiogenesis, Vascular Biology
Shipping TypeBlue ice
Storage-20°C
Storage / StabilityHuman
More Information
Suggested Application

Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
  • Introduction

    RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

    This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

    Product Features

    • High sensitivity (pg/mL to ng/mL levels)
    • High specificity and system reproducibility
    • Suitable for diverse sample types
    • Low sample volume requirement: 50 µL or less
    • Get results same day (6-hour processing time)
    • Well-suited for high throughput assays
    • Test 16 samples on each slide
    • Data analysis software provides one-step computation

    Target Names

    Scroll over each target protein for more information
    Activin A
    • Gene Symbol: INHBA
    • Gene ID: 3624
    • Accession #: P08476
    • ELISA Kit: RayBio Human Activin A ELISA
    AgRP
    • Gene Symbol: AgRP
    • Gene ID: 181
    • Accession #: O00253
    • ELISA Kit: RayBio Human AgRP ELISA
    Angiopoietin-1
    • Gene Symbol: ANGPT1
    • Gene ID: 284
    • Accession #: Q15389
    • ELISA Kit: RayBio Human ANGPT1 ELISA
    Angiopoietin-2
    • Gene Symbol: ANGPT2
    • Gene ID: 285
    • Accession #: O15123
    • ELISA Kit: RayBio Human ANGPT2 ELISA
    Angiogenin
    • Gene Symbol: ANG
    • Gene ID: 283
    • Accession #: P03950
    • ELISA Kit: RayBio Human Angiogenin ELISA
    Angiostatin
    • Gene Symbol: PLG
    • Gene ID: 5340
    • Accession #: P00747
    • ELISA Kit: RayBio Human Angiostatin ELISA
    ANGPTL4
    • Gene Symbol: ANGPTL4
    • Gene ID: 51129
    • Accession #: Q9BY76
    • ELISA Kit: RayBio Human ANGPTL4 ELISA
    bFGF
    • Gene Symbol: FGF2
    • Gene ID: 2247
    • Accession #: P09038
    • ELISA Kit: RayBio Human bFGF ELISA
    CXCL16
    • Gene Symbol: CXCL16
    • Gene ID: 58191
    • Accession #: Q9H2A7
    • ELISA Kit: RayBio Human CXCL16 ELISA
    EGF
    • Gene Symbol: EGF
    • Gene ID: 1950
    • Accession #: P01133
    • ELISA Kit: RayBio Human EGF ELISA
    ENA-78
    (CXCL5)
    • Gene Symbol: CXCL5
    • Gene ID: 6374
    • Accession #: P42830
    • ELISA Kit: RayBio Human ENA-78 ELISA
    FGF-4
    • Gene Symbol: FGF4
    • Gene ID: 2249
    • Accession #: P08620
    • ELISA Kit: RayBio Human FGF-4 ELISA
    Follistatin
    • Gene Symbol: FST
    • Gene ID: 10468
    • Accession #: P19883
    • ELISA Kit: RayBio Human Follistatin ELISA
    GCSF
    • Gene Symbol: CSF3
    • Gene ID: 1440
    • Accession #: P09919
    • ELISA Kit: RayBio Human G-CSF ELISA
    GM-CSF
    • Gene Symbol: CSF2
    • Gene ID: 1437
    • Accession #: P04141
    • ELISA Kit: RayBio Human GM-CSF ELISA
    GRO alpha/beta/gamma
    HB-EGF
    • Gene Symbol: HBEGF
    • Gene ID: 1839
    • Accession #: Q99075
    • ELISA Kit: RayBio Human HB-EGF ELISA
    HGF
    • Gene Symbol: HGF
    • Gene ID: 3082
    • Accession #: P14210
    • ELISA Kit: RayBio Human HGF ELISA
    I-309
    (TCA-3/CCL1)
    • Gene Symbol: CCL1
    • Gene ID: 6346
    • Accession #: P22362
    • ELISA Kit: RayBio Human I-309 ELISA
    IFN-gamma
    • Gene Symbol: IFNG
    • Gene ID: 3458
    • Accession #: P01579
    • ELISA Kit: RayBio Human IFN-gamma ELISA
    IGF-1
    • Gene Symbol: IGF1
    • Gene ID: 3479
    • Accession #: P05019
    • ELISA Kit: RayBio Human IGF-1 ELISA
    IL-10
    • Gene Symbol: IL10
    • Gene ID: 3586
    • Accession #: P22301
    • ELISA Kit: RayBio Human IL-10 ELISA
    IL-12 p40
    • Gene Symbol: IL12B
    • Gene ID: 3593
    • Accession #: P29460
    • ELISA Kit: RayBio Human IL-12 p40 ELISA
    IL-12 p70
    • Gene Symbol: IL12A
    • Gene ID: 3592
    • Accession #: P29459
    • ELISA Kit: RayBio Human IL-12 p70 ELISA
    IL-17A
    • Gene Symbol: IL17A
    • Gene ID: 3605
    • Accession #: Q16552
    • ELISA Kit: RayBio Human IL-17A ELISA
    IL-1 alpha
    (IL-1 F1)
    • Gene Symbol: IL1A
    • Gene ID: 3552
    • Accession #: P01583
    • ELISA Kit: RayBio Human IL-1 alpha ELISA
    IL-1 beta
    (IL-1 F2)
    • Gene Symbol: IL1B
    • Gene ID: 3553
    • Accession #: P01584
    • ELISA Kit: RayBio Human IL-1 beta ELISA
    IL-2
    • Gene Symbol: IL2
    • Gene ID: 3558
    • Accession #: P60568
    • ELISA Kit: RayBio Human IL-2 ELISA
    IL-4
    • Gene Symbol: IL4
    • Gene ID: 3565
    • Accession #: P05112
    • ELISA Kit: RayBio Human IL-4 ELISA
    IL-6
    • Gene Symbol: IL6
    • Gene ID: 3569
    • Accession #: P05231
    • ELISA Kit: RayBio Human IL-6 ELISA
    IL-8
    (CXCL8)
    • Gene Symbol: IL8
    • Gene ID: 3576
    • Accession #: P10145
    • ELISA Kit: RayBio Human IL-8 ELISA
    IP-10
    (CXCL10)
    • Gene Symbol: CXCL10
    • Gene ID: 3627
    • Accession #: P02778
    • ELISA Kit: RayBio Human IP-10 ELISA
    I-TAC
    (CXCL11)
    • Gene Symbol: CXCL11
    • Gene ID: 6373
    • Accession #: O14625
    • ELISA Kit: RayBio Human I-TAC ELISA
    Leptin
    • Gene Symbol: LEP
    • Gene ID: 3952
    • Accession #: P41159
    • ELISA Kit: RayBio Human Leptin ELISA
    LIF
    • Gene Symbol: LIF
    • Gene ID: 3976
    • Accession #: P15018
    • ELISA Kit: RayBio Human LIF ELISA
    MCP-1
    (CCL2)
    • Gene Symbol: CCL2
    • Gene ID: 6347
    • Accession #: P13500
    • ELISA Kit: RayBio Human MCP-1 ELISA
    MCP-2
    (CCL8)
    • Gene Symbol: CCL8
    • Gene ID: 6355
    • Accession #: P80075
    • ELISA Kit: RayBio Human MCP-2 ELISA
    MCP-3
    (MARC/CCL7)
    • Gene Symbol: CCL7
    • Gene ID: 6354
    • Accession #: P80098
    • ELISA Kit: RayBio Human MCP-3 ELISA
    MCP-4
    (CCL13)
    • Gene Symbol: CCL13
    • Gene ID: 6357
    • Accession #: Q99616
    • ELISA Kit: RayBio Human MCP-4 ELISA
    MMP-1
    • Gene Symbol: MMP1
    • Gene ID: 4312
    • Accession #: P03956
    • ELISA Kit: RayBio Human MMP-1 ELISA
    MMP-9
    • Gene Symbol: MMP9
    • Gene ID: 4318
    • Accession #: P14780
    • ELISA Kit: RayBio Human MMP-9 ELISA
    PDGF-BB
    • Gene Symbol: PDGFB
    • Gene ID: 5155
    • Accession #: P01127
    • ELISA Kit: RayBio Human PDGF-BB ELISA
    PECAM-1
    (CD31)
    • Gene Symbol: PECAM1
    • Gene ID: 5175
    • Accession #: P16284
    • ELISA Kit: RayBio Human PECAM-1 ELISA
    PLGF
    • Gene Symbol: PGF
    • Gene ID: 5228
    • Accession #: P49763
    • ELISA Kit: RayBio Human PLGF ELISA
    RANTES
    (CCL5)
    • Gene Symbol: CCL5
    • Gene ID: 6352
    • Accession #: P13501
    • ELISA Kit: RayBio Human RANTES ELISA
    TGF alpha
    • Gene Symbol: TGFA
    • Gene ID: 7039
    • Accession #: P01135
    • ELISA Kit: RayBio Human TGF-alpha ELISA
    TGF beta 1
    • Gene Symbol: TGFB1
    • Gene ID: 7040
    • Accession #: P01137
    • ELISA Kit: RayBio Human TGF-beta 1 ELISA
    TGF beta 3
    • Gene Symbol: TGFB3
    • Gene ID: 7043
    • Accession #: P10600
    Tie-1
    • Gene Symbol: TIE1
    • Gene ID: 7075
    • Accession #: P35590
    • ELISA Kit: RayBio Human Tie-1 ELISA
    Tie-2
    • Gene Symbol: TEK
    • Gene ID: 7010
    • Accession #: Q02763
    • ELISA Kit: RayBio Human Tie-2 ELISA
    TIMP-1
    • Gene Symbol: TIMP1
    • Gene ID: 7076
    • Accession #: P01033
    • ELISA Kit: RayBio Human TIMP-1 ELISA
    TIMP-2
    • Gene Symbol: TIMP2
    • Gene ID: 7077
    • Accession #: P16035
    • ELISA Kit: RayBio Human TIMP-2 ELISA
    TNF alpha
    • Gene Symbol: TNF
    • Gene ID: 7124
    • Accession #: P01375
    • ELISA Kit: RayBio Human TNF-alpha ELISA
    TNF beta
    (TNFSF1B)
    • Gene Symbol: LTA
    • Gene ID: 4049
    • Accession #: P01374
    • ELISA Kit: RayBio Human TNF-beta ELISA
    Thrombopoietin
    (TPO)
    • Gene Symbol: THPO
    • Gene ID: 7066
    • Accession #: P40225
    • ELISA Kit: RayBio Human TPO ELISA
    uPAR
    • Gene Symbol: PLAUR
    • Gene ID: 5329
    • Accession #: Q03405
    • ELISA Kit: RayBio Human uPAR ELISA
    VEGF-A
    • Gene Symbol: VEGFA
    • Gene ID: 7422
    • Accession #: P15692
    • ELISA Kit: RayBio Human VEGF-A ELISA
    VEGFR2
    • Gene Symbol: KDR
    • Gene ID: 3791
    • Accession #: P35968
    • ELISA Kit: RayBio Human VEGF R2 ELISA
    VEGFR3
    • Gene Symbol: FLT4
    • Gene ID: 2324
    • Accession #: P35916
    • ELISA Kit: RayBio Human VEGF R3 ELISA
    VEGF-D
    • Gene Symbol: FIGF
    • Gene ID: 2277
    • Accession #: O43915
    • ELISA Kit: RayBio Human VEGF-D ELISA

    Species Detected

    Human

    Application Notes

    Suggested Application
    Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
  • Kit Components
    • Antibody Array Slide(s)
    • Blocking Buffer
    • Wash Buffer 1
    • Wash Buffer 2
    • Lyophilized Standard Mix
    • Biotinylated Detection Antibody Cocktail
    • Streptavidin-Conjugated Fluor
    • 30 ml Centrifuge Tube
    • Adhesive Plastic Strips
    • Manual
    Other Materials Required
    • Distilled or deionized water
    • Small plastic boxes or containers
    • Pipettors, pipette tips and other common lab consumables
    • Orbital shaker or oscillating rocker
    • Aluminum foil
    • Gene microarray scanner or similar laser fluorescence scanner

    View Compatible Laser Scanners

    Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
    Protocol Outline
    1. Dry the glass slide
    2. Prepare Standards
    3. Block array surface
    4. Incubate with Samples and Standards
    5. Incubate with Biotinylated Detection Antibody Cocktail
    6. Incubate with Streptavidin-Conjugated Fluor
    7. Disassemble the glass slide
    8. Scan with a gene microarray laser scanner
    9. Perform densitometry and analysis

    Storage/Stability

    For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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