RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.
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Figure adapted from Huang et al., Bioactive Materials, 2023.
Human Cytokine Array C1000
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 120 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Adiponectin (ACRP30)
| AgRP
| Amphiregulin
| Angiogenin
| Angiopoietin-2
|
| Axl
| BDNF
| bFGF
| BLC (CXCL13)
| BMP-4
|
| BMP-6
| beta-NGF
| Betacellulin (BTC)
| CCL28 (MEC)
| CK beta 8-1 (CCL23)
|
| CNTF
| CTACK (CCL27)
| Dtk
| EGF
| EGFR
|
| ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| Fas (TNFRSF6/Apo-1)
|
| FGF-4
| FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
|
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
| GCSF
| GDNF
| GITR (TNFRSF18)
|
| GITR Ligand (TNFSF18)
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
|
| HGF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| ICAM-3 (CD50)
| IFN-gamma
|
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
|
| IGF-1
| IGF-1 R
| IL-1 R4 (ST2)
| IL-1 R1
| IL-10
|
| IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
| IL-16
| IL-17A
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
| IL-2
| IL-2 R alpha
| IL-3
| IL-4
| IL-5
|
Typical Data
Kit Components
- Human Cytokine Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
Excel (AAH-CYT-6_Analysis_Tool.xls)
Excel (AAH-CYT-7_Analysis_Tool.xls)
citations
LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration
- Year:
- 2018
- Journal Name:
- Nature
- PubMed ID:
- 30333625
Macrophage-Targeted Lipid Nanoparticle Delivery of microRNA-146a to Mitigate Hemorrhagic Shock-Induced Acute Respiratory Distress Syndrome
- Species:
- Human
- Sample Type:
- Human primary alveolar macrophages (AMs)
- Year:
- 2023
- Journal Name:
- Acs Nano
Neuroplasticity therapy using glia-like cells derived from human mesenchymal stem cells for the recovery of cerebral infarction sequelae
- Year:
- 2025
- Journal Name:
- Molecular Therapy
- PubMed ID:
- 39563032
Insulin Receptor Substrate-2 Regulates the Secretion of Growth Factors in Response to Amino Acid Deprivation
- Year:
- 2025
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39859555
IL1R2 Blockade Alleviates Immunosuppression and Potentiates Anti-PD-1 Efficacy in Triple-Negative Breast Cancer
- Year:
- 2024
- Journal Name:
- Cancer Research
- PubMed ID:
- 38657120
JCPyV infection of primary choroid plexus epithelial cells reduces expression of critical junctional proteins and increases expression of barrier disrupting inflammatory cytokines
- Year:
- 2024
- Journal Name:
- Microbiol Spectr
- PubMed ID:
- 38874395
CTHRC1 promotes colorectal cancer progression by recruiting tumor-associated macrophages via up-regulation of CCL15
- Year:
- 2024
- Journal Name:
- J Mol Med (Berl)
- PubMed ID:
- 37987774
Chemotherapy-induced PDL-1 expression in cancer-associated fibroblasts promotes chemoresistance in NSCLC
- Species:
- Human
- Sample Type:
- Cancer-associated fibroblasts (CAFs)
- Year:
- 2023
- Journal Name:
- Lung Cancer
Infrapatellar Fat Pad Modulates Osteoarthritis-Associated Cytokine and MMP Expression in Human Articular Chondrocytes
- Year:
- 2023
- Journal Name:
- Cells
- PubMed ID:
- 38132170
Reciprocal Interaction of Cancer Stem Cells of Cholangiocarcinoma with Macrophage
- Species:
- Human
- Sample Type:
- HuCC-T1 cell
- Year:
- 2023
- Journal Name:
- Stem Cell Reviews and Reports