Human DEFa6 (Defensin Alpha 6, Paneth Cell Specific) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	DEFA6
Gene ID:	1671
Uniprot ID:	DEF6_HUMAN
Immunogen Region:	Ready-To-Use
Sample Type:	serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed in Paneth cells of the small intestine (at protein level).
Post Translational Modifications	Proteolytically cleaved by trypsin at Arg-68.the propeptide is stored in the tissue of the small intestine and the mature peptide is found in the luminal fluid.cleavage may occur during or after release into the lumen. The N-terminal propeptide region suppresses self-assembly and renders DEFA6 propeptide unable to agglutinate bacteria and protect human epithelial cells from bacterial invasion. Under reducing conditions, naturally present in the gut owing to the low redox potential or enzymatically generated by the thioredoxin system, the disulfide bridges are opened leading to a conformational change of DEF6, thereby changing its antimicrobial spectrum. The reduced form exhibits inhibitory activity against anaerobic bacteria, in contrast to the minimal antimicrobial activity of the disulfide-linked oxidized form. The formation of higher-order nanonets and bacterial entrapment is independent of the redox state.
Function	Host-defense peptide that contributes to intestinal innate immunity and mediates homeostasis at mucosal surfaces by forming higher-order oligomers that capture bacteria and prevent microbial invasion of the epithelium. After binding to bacterial surface proteins, undergoes ordered self-assembly to form fibril-like nanonets that surround and entangle bacteria and thereby prevent bacterial invasion across the epithelial barrier. Entangles and agglutinates Gram-negative bacteria, such as E.coli, S.typhimurium and Y.enterocolitica, and Gram-positive bacteria such as L.monocytogenes, thereby protecting the intestine against invasion by enteric bacterial pathogens. Blocks adhesion of C.albicans to intestinal epithelial cells and thereby suppresses fungal invasion of epithelial cells and biofilm formation. Under reducing conditions and in an acidic environment similar to the intestinal milieu, exhibits inhibitory activity against anaerobic bacteria such as B.adolescentis, L.acidophilus and B.breve, as well as B.longum and S.thermophilus, possibly by leading to alterations in bacterial cell envelope structures. The disulfide-linked oxidized form exhibits negligible antimicrobial activity against Gram-negative and Gram-positive bacteria, as compared to the enteric defensin DEFA5.
Protein Name	Defensin-6 Defensin - Alpha 6
Database Links	Reactome: R-HSA-1461973 Reactome: R-HSA-1462054
Cellular Localisation	Secreted Cytoplasmic Vesicle Secretory Vesicle Stored As Propeptide In Secretory Granules Of Small Intestinal Paneth Cells And Found In The Ileum Lumen As Mature Peptide
Alternative ELISA Names	Defensin-6 ELISA kit Defensin - Alpha 6 ELISA kit DEFA6 ELISA kit DEF6 ELISA kit
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Information sourced from Uniprot.org

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