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> Human GDF-15 ELISA Kit

Human GDF-15 ELISA Kit

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Cat# ELH-GDF15-1

Size : 1PlateKit

Brand : RayBiotech


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Human GDF-15 ELISA Kit Standard CurveHuman GDF-15 ELISA KitHuman GDF-15 ELISA KitThe Human GDF15 ELISA Kit was used to quantify elevated plasma GDF15 levels in tumor-bearing mice in a cancer cachexia model. Figure adapted from Khatri et al., Cancer Letters, 2025.The Human GDF15 ELISA Kit was used to quantify cerebrospinal fluid GDF15 levels across varying stages of HIV-associated neurocognitive impairment. Figure adapted from Boustani et al., Brain Sciences, 2025.The Human GDF15 ELISA Kit was used to quantify serum GDF15 levels in gastric cancer patients, which were used to stratify patients for overall survival analysis. Figure adopted from Wang et al., Cancer Science, 2023.The Human GDF15 ELISA Kit was used to quantify plasma GDF-15 levels across stages of diabetic kidney disease, demonstrating increased levels with worsening albuminuria. Figure adapted from Hussain et al., Diabetes Research and Clinical Practice, 2020.The kit was used to quantify serum GDF15 levels in patients with non-small-cell lung carcinoma, confirming significantly elevated concentrations compared to healthy controls. Figure adapted from Cai et al., Cytokine, 2020.Human GDF-15 ELISA KitHuman GDF-15 ELISA KitHuman GDF-15 ELISA Kit5 Plate Kit Packaging (one box)

Human GDF-15 ELISA Kit Standard Curve

Human GDF-15 ELISA Kit

RayBio® Human GDF-15 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 800 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
3 - 30 fold
Accession Number
Q99988
Gene Id
9518
Gene Symbols
GDF15, PDF, MIC1, PLAB, PTGFB
Protein Name / Synonyms
Growth/differentiation factor 15 (GDF-15) (Macrophage inhibitory cytokine 1) (MIC-1) (NSAID-activated gene 1 protein) (NAG-1) (NSAID-regulated gene 1 protein) (NRG-1) (Placental TGF-beta) (Placental bone morphogenetic protein) (Prostate differentiation factor)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Shipping TypeBlue ice
Storage≤-20°C

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Background

Growth/differentiation factor 15 (GDF-15) is a divergent member of the TGF-β superfamily of proteins (aliases: MIC-1, NAG-1, pTGFB, PDF and PLAB). Mature GDF-15 is released as a homodimer where each dimer is 25 kDa. The expression of GDF-15 is most prominent in the placenta, followed by the prostrate. Outside of the reproductive organs, it shows low constitutive expression, however, expression can be induced by stress. Cell types that produce GDF-15 include, cardiomyocytes, adipocytes, macrophages, endothelial cells and vascular smooth muscle cells. As a stress-induced cytokine, it is regulated by inflammation and stress-related proteins. The effects of GDF-15 are seen during tissue injury, metabolic conditions, pregnancy (immune tolerance) and various cancers.

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

The Human GDF15 ELISA Kit was used to quantify elevated plasma GDF15 levels in tumor-bearing mice in a cancer cachexia model. Figure adapted from Khatri et al., Cancer Letters, 2025.

The Human GDF15 ELISA Kit was used to quantify cerebrospinal fluid GDF15 levels across varying stages of HIV-associated neurocognitive impairment. Figure adapted from Boustani et al., Brain Sciences, 2025.

The Human GDF15 ELISA Kit was used to quantify serum GDF15 levels in gastric cancer patients, which were used to stratify patients for overall survival analysis. Figure adopted from Wang et al., Cancer Science, 2023.

The Human GDF15 ELISA Kit was used to quantify plasma GDF-15 levels across stages of diabetic kidney disease, demonstrating increased levels with worsening albuminuria. Figure adapted from Hussain et al., Diabetes Research and Clinical Practice, 2020.

The Human GDF15 ELISA Kit (ELH-GDF15) was used to quantify serum GDF15 levels in patients with non-small-cell lung carcinoma, confirming significantly elevated concentrations compared to healthy controls. Figure adapted from Cai et al., Cytokine, 2020.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

documents

Datasheet (ELH-GDF15.pdf)
SDS (ELH-GDF15.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Selpercatinib mitigates cancer cachexia independent of anti-tumor activity in the HT1080 tumor model

    Year:
    2025
    Journal Name:
    Cancer Letters
    PubMed ID:
    39778760

  • Increased Growth Differentiation Factor 15 Levels Are Associated with HIV-Associated Neurocognitive Impairment: A Pilot Study

    Year:
    2025
    Journal Name:
    Brain Sci
    PubMed ID:
    39851417

  • Moderate-intensity constant or high-intensity interval training Metabolic effects on candidates to undergo bariatric surgery

    Year:
    2024
    Journal Name:
    Nutr Metab Cardiovasc Dis
    PubMed ID:
    38553359

  • Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Cancer Science

  • Conditioned Media from Head and Neck Cancer Cell Lines and Serum Samples from Head and Neck Cancer Patients Drive Catabolic Pathways in Cultured Muscle Cells

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Cancers

  • Hyperactive behaviour of growth differentiation factor- 15 (GDF-15) in conjunction with iron trafficking transporters and suppression of Nrf-2 gene in diabetes and metabolic syndrome

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Molecular and Cellular Biochemistry

  • Prognostic value of interleukin-34 and interleukin-38 in patients with newly diagnosed atrial fibrillation

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Frontiers in cardiovascular medicine

  • Cell non-autonomous effect of hepatic growth differentiation factor 15 on the thyroid gland

    Year:
    2022
    Journal Name:
    Frontiers in Endocrinology
    PubMed ID:
    36046792

  • Potential biomarkers for early detection of diabetic kidney disease

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2020
    Journal Name:
    Diabetes Research and Clinical Practice

  • Extensive serum biomarker analysis in patients with non-small-cell lung carcinoma

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2020
    Journal Name:
    Cytokine

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