Human Insulin ELISA Kit

Cat# ELH-Insulin-1

Size : 1PlateKit

Brand : RayBiotech


Human Insulin ELISA Kit

RayBio® Human Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
4 µIU/ml

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Detection Range
4 µIU/ml - 300 µIU/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 - 10 fold
Accession Number
P01308
Gene Id
3630
Gene Symbols
INS
Protein Name / Synonyms
Insulin [Cleaved into: Insulin B chain Insulin A chain]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaInflammation, Antibody Drug Development, Protein Therapeutics
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Insulin is a polypeptide hormone, secreted by β-cells in the islets of Langerhans of the pancreas. It plays a crucial role in glucose homeostasis, cell growth and metabolism. Upon secretion, insulin circulates to hepatocytes where glucose is stored in the form of glycogen. Other targets of insulin include skeletal muscles and adipocytes which also take up glucose, thereby reducing blood glucose levels. Insulin triggers glucose uptake, muscle protein synthesis, glycogenesis and lipogenesis via the tyrosine kinase pathway. In addition to its well-studied role in diabetes, insulin acts on key organs in the body, including the brain, heart, kidneys, bone and skin.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-Insulin-1 was used to measure plasma insulin levels in mouse models of metabolic syndrome treated with asprosin-neutralizing antibodies. Figure adapted from Mishra et al., eLife, 2021.


ELH-Insulin was used to quantify glucose-stimulated insulin secretion from human iPSC-derived pancreatic organoids cultured in GACO scaffolds. Figure adapted from Sandilya & Singh, Cell Regeneration, 2021.


ELH-Insulin was used to measure circulating insulin levels before and after unilateral resistance training at multiple time points. Figure adapted from Beyer et al., J Strength Cond Res, 2016.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

documents

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • LncRNA NEAT1 and MALAT1 are involved in polycystic ovary syndrome pathogenesis by functioning as competing endogenous RNAs to control the expression of PCOS-related target genes

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Non-coding RNA research
  • Resilience-based Islamic program as a promising intervention on diabetes fatigue and health-related quality of life

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Plos One
  • Effect of High-Intensity Interval Training Combined with Blood Flow Restriction at Different Phases on Abdominal Visceral Fat among Obese Adults: A Randomized Controlled Trial

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    International Journal of Environmental Research and Public Health
  • Inflammatory Status and Cardio-metabolic Risk Stratification of Rotational Shift Work

    Year:
    2022
    Journal Name:
    Ann Work Expo Health
    PubMed ID:
    34219158
  • Asprosin-neutralizing antibodies as a treatment for metabolic syndrome

    Year:
    2021
    Journal Name:
    eLife
    PubMed ID:
    33904407
  • Exploring protein stabilized multiple emulsion with permeation enhancer for oral delivery of insulin

    Year:
    2021
    Journal Name:
    International Journal of Biological Macromolecules
    PubMed ID:
    33279562
  • Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold

    Year:
    2021
    Journal Name:
    Cell Regen
    PubMed ID:
    34850295
  • Kuche, et al., Exploring protein stabilized multiple emulsion with permeation enhancer for oral delivery of insulin, International Journal of Biological Macromolecules (2018)

    Species:
    Human
    Sample Type:
    Plasma (plasma insulin level)
    Year:
    2020
    Journal Name:
    International Journal of Biological Macromolecules
  • Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2020
    Journal Name:
    Tissue Engineering and Regenerative Medicine
  • Exercise training-induced visceral fat loss in obese women: The role of training intensity and modality

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2020
    Journal Name:
    Scandanavian Journal of Medicine and Science in Sports

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