Human Lambda, Mouse ads. Antibody : AP
Cat# OASB01879
Size : 1.0mL
Brand : Aviva Systems Biology
Goat Anti-Human Lambda Antibody - AP Conjugated (OASB01879)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Human Lambda Antibody - AP Conjugated (OASB01879) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | AP |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with human lambda light chains; purified by affinity chromatography on human lambda light chains covalently linked to agarose; reacts with human lambda light chains; cross-adsorbed against human kappa light chains / mouse immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human lambda light chains |
| Purification | Affinity chromatography on pooled human Igs with λ light chains covalently linkedto agarose |
| Concentration | Lot specific |
| Specificity | Lambda |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent. |
| Dilution | Immunofluorescence ELISA: Purified antibody Purified antibody AP conjugate <= 1 ug/106 cells 1ug/mL 1:2,000-1:4,000 |
| Application Info | Direct immunofluorescence staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mMboratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C. |
| Cross Absorption | Pooled human myeloma proteins with κ light chains and with mouseimmunoglobulins. |
| Gene Symbol | IGL |
|---|---|
| Gene Full Name | Immunoglobulin lambda-like polypeptide 1 |
| Alias Symbols | IGL@, IGLC6 |
| NCBI Gene Id | 3535 |
| Protein Name | ig lambda-6 chain C region |
| Description of Target | Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. |
