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IgG ELISA Kit (Cat)

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Cat# OKIA00014

Size : 1x96wellplate

Brand : Aviva Systems Biology


IgG ELISA Kit (Cat) (OKIA00014)

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Standard curve
Datasheets/ManualsPrintable datasheet for IgG ELISA Kit (Cat) (OKIA00014)
Product Info
Predicted Species ReactivityCat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration80 Minutes
ELISA Kit PrincipleAviva Systems Biology IgG ELISA Kit (Cat) (OKIA00014) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG captured in the well.
ELISA Kit Range12.5 ng/ml - 800 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Cat IgG Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Cat IgG Calibrator1
Additional InformationIntended Use: The IgG test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in biological samples of cats.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Cat IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-CAT IgG ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Cat IgG.
7. CAT IgG CALIBRATOR
One vial containing Cat IgG calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity4.095 ng/ml
Predicted Homology Based on Immunogen SequenceCat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsIGHG1, Ig gamma, Immunoglobulin G, Ig gamma-1 chain C region
Protein NameIgG